Cyanotoxin Sampling
Sampling Details
- Handling – follow collection and handling procedures established by method or laboratory
- Lab Analysis – use lab-provided sample containers
- Containers – typically amber glass
- Quenching – quench (usually with sodium thiosulfate) immediately upon sampling if exposed to oxidants
- Cooling – cool on ice (4° C) immediately after collection, during shipping, and pending analysis
- Holding Time – analyze within 5 days of collection
- More Info – USGS Guidelines for Design and Sampling for Cyanobacterial Toxin and Taste-and-Odor Studies in Lakes and Reservoirs (2008)
Acceptable Analytical Methods for Cyanotoxins
Microcystins
Analytical Method | Source Water | Raw Water | Finished Water – Investigation | Finished Water – Compliance |
---|---|---|---|---|
ELISA Cylindrospermopsin Method | Acceptable | Acceptable | Acceptable | Acceptable |
LC/ESI-MS/MS Method 545 | Acceptable | Acceptable | Acceptable | ? |
Immunochromatographic Strip Test for Detection of Cylindrospermopsin | Acceptable | Acceptable | Acceptable | Not Acceptable |
Cylindrospermopsin
Analytical Method | Source Water | Raw Water | Finished Water – Investigation | Finished Water – Compliance |
---|---|---|---|---|
ELISA Cylindrospermopsin Method | Acceptable | Acceptable | Acceptable | Acceptable |
LC/ESI-MS/MS Method 545 | Not Acceptable | Not Acceptable | Acceptable | Acceptable |
Immunochromatographic Strip Test for Detection of Cylindrospermopsin | Acceptable | Acceptable | Acceptable | Not Acceptable |
Saxitoxins
Analytical Method | Source Water | Raw Water | Finished Water – Investigation | Finished Water – Compliance |
---|---|---|---|---|
ELISA Saxitoxin Method | Acceptable | Acceptable | Acceptable | Acceptable |
LC-MS/MS Method for detection of 12 saxitoxin variants | Acceptable | Acceptable | Acceptable | Acceptable |
Anatoxin-a
Analytical Method | Source Water | Raw Water | Finished Water – Investigation | Finished Water – Compliance |
---|---|---|---|---|
LC/ESI-MS/MS Method 545 | Not Acceptable | Not Acceptable | Acceptable | Acceptable |
Immunochromatographic Strip Test for Detection of Anatoxin-a | Acceptable | Acceptable | Acceptable | Not Acceptable |